Biofilm form when bacteria attach to wetted surfaces, and begin to excrete a slimy, glue-like substance. Colonies of biofilm bacteria carry out a variety of detrimental or beneficial reactions that affect all of us daily.
At the Center for Biofilm Engineering (CBE), multidisciplinary research teams find solutions and applications for industrially relevant problems and potentials of microbial biofilm formation. The CBE was established in 1990 as a National Science Foundation Engineering Research Center to foster a new approach to university engineering and science education.
Molecular Biosciences Program
612 Leon Johnson Hall
P.O. Box P.O. Box 172580,
Bozeman, MT 59717 4069946652 Fax: 4069947212 firstname.lastname@example.org
Pseudomonas aeruginosa biofilms and virulence factors
The Franklin lab has three primary areas of research regarding the activity of the oppportunistic pathogen Pseudomonas aeruginosa.
(I) Biosynthesis of the protective capsular polysaccharide.
(II) Spatial and Temporal aspects of gene expression in P. aeruginosa biofilms.
(III) Interactions of P. aeruginosa with the innate immune response.
Stewart, P.S. and M.J. Franklin (2008) Physiological heterogeneity in biofilms. Nature Reviews Microbiology 6:199-210.
Lenz, A.P., K. Williamson, B. Pitts, P.S. Stewart, and M.J. Franklin (2008) Localized gene expression in Pseudomonas aeruginosa biofilms Appl.
Environ. Microbiol. 74: 4463–4471.
Lenz, A.P. and M.J. Franklin (2008) qRT-PCR of Microbial Biofilms. Cold Spring Harbor Protocols doi:10.1101/pdb.prot5066.
Lenz, A.P. and M.J. Franklin (2008) Isolation of RNA and DNA from biofilm samples obtained by Laser Capture Microdissection Microscopy. Cold Spring Harbor Protocols doi:10.1101/pdb.prot5065.
Patrauchan, M.A., S. Sarkisova, and M.J. Franklin (2007)
Strain-specific proteome responses of Pseudomonas aeruginosa to biofilm growth and to calcium. Microbiology 153: 3838-3851