We are interested in environmental signals that are sensed by cells to mediate control over physiology and modes of growth. In particular, we are interested in the genes used to sense environmental changes in response to biotic and abiotic parameters, and how microibal cells respond in order to optimize metabolism. We study both monocultures and indigenous microbial communities to better understand the interrelationships between genomic content and phenotype at different levels of resolution (i.e., DNA to communinty), and how these attributes contribute to stress and survival of biological cells. Within the contexts of cellular responses, we study bacterial systems important for heavy metal biormediation, metal corrosion, extemophilic lifestyles, and bio-energy.
Clark, M.E., Q. He, Z. He, E.J. Alm, K.H. Huang, T.C. Hazen, A.P. Arkin, J.D. Wall, J. Zhou, and M.W. Fields. 2006. Temporal transcriptomic analyses of Desulfovibrio vulgaris Hildenborough during electron donor depletion. Appl. Environ. Microbiol. 72:5578-5588.
Fields, M.W., C.E. Bagwell, S.L. Carroll, T. Yan, X. Liu, D.B. Watson, P.M. Jardine, C.S.Criddle, T.C. Hazen, and J. Zhou. 2006. Phylogenetic and functional biomakers as indicators of bacterial community responses to mixed-waste contamination. Environ. Sci. Technol. 40:2601-2607.
Hwang, C., W.-M.Wu, T.J. Gentry, J. Carley, S.L. Carroll, C. Schadt, D. Watson, P.M. Jardine, J. Zhou, R.F. Hickey, C.S. Criddle, and M.W. Fields. 2006. Changes in bacterial community structure correlate with initial operating conditions of a field-scale denitrifying fluidized bed reactor. Appl. Microbiol. Biotech. 71:748-760.
Gao, W., Y. Liu, C.S. Giometti, S.L. Tollaksen, T. Khare, L. Wu, D.M. Klingeman, M.W. Fields and J. Zhou. 2006. Knock-out of a prohibitin-like protein results in alteration of iron metabolism, increased spontaneous mutation and hydrogen peroxide sensitivity in the bacterium Shewanella oneidensis. BMC Genomics 7:76
Fields, M.W., T. Yan, S.-K. Rhee, S.L. Carroll, P.M. Jardine, D.B. Watson, C.S. Criddle and J. Zhou. 2005. Impacts on microbial communities and cultivable isolates from groundwater contaminated with high levels of nitric acid-uranium waste. FEMS Microboil. Ecol. 53:417-428