Dr. Dlakic holds Ph.D. in Biochemistry from University of Nevada, Reno. His research uses multi-disciplinary approaches by combining Biochemistry, Cell and Molecular Biology with Bioinformatics.
Dr. Dlakic's interest fall into two complementary categories. He works on establishing links between structurally characterized proteins and protein families that show are functionally uncharacterized at present. This "predictive structural biology" relies on structural and mechanistic conservation between proteins to assign functions to unknown proteins. The following two pages contain more information about this work:
The second line of research uses variety of experimental methods to study molecular functions of eukaryotic proteins involved in ribosomal synthesis and assembly. Ribosome synthesis in eukaryotes requires a fine-tuned molecular assembly line where tasks are performed sequentially by many auxiliary proteins. Even though most trans-acting proteins have been identified, much less is known about the order of assembly steps, the exact cellular localization of RNA-modifying and scaffolding complexes, and about regulatory mechanisms that ensure precise coordination of all events. Dr. Dlakic uses flourescence complementation to study spatial and temporal distribution of proteins and their interactions which contribute to ribosome assembly (see http://ribosome.dlakiclab.org/ for more information).
Fatica, A., Cronshaw, A. D., Dlakic, M. and Tollervey, D. (2002) Ssf1p Prevents Premature Processing of an Early pre-60S Ribosomal Particle. Mol. Cell, 9, 341-351.
Oeffinger, M. Dlakic, M. and Tollervey, D. (2004) A pre-ribosome associated HEAT-repeat protein is required for export of both ribosomal subunits. Genes Dev. 18, 196-209.
Fatica, A., Tollervey, D. and Dlakic, M. (2004) PIN domain of Nob1p is required for D-site cleavage in 20S pre-rRNA. RNA 10, 1698-1701.
Dlakic M (2006) DUF283 Domain of Dicer Proteins Has a Double-Stranded RNA-Binding Fold. Bioinformatics 22, 2711-2714.
Dlakic M (2009) HHsvm: Fast and accurate classification of profile-profile matches identified by HHsearch. Bioinformatics 25, 3071-3076.